Nanoscopy and Multidimensional Optical Fluorescence Microscopy

The highlight of the book is its coverage of optical nanoscopy and super-resolution microscopy. The rapid advances in this area over the past few years offer researchers in both photonics and molecular biologya wealth of accomplishment upon which they can build.

Offering a complete treatment of this emerging field, this volume —

Describes how scientists have exploited the properties of light and its fluorophore partners to overcome the resolution limit of conventional light microscopy

Delves into recent ways to minimize the photobleaching that has long hampered many methods including those that have the potential to capture previously unobtainable information on the movements of single molecules

Discusses the principles, benefits, and implementation of fluorescence correlation spectroscopy and related methods, which simplifies analysis by limiting light to stationary focal points in a sample

Considers the most basic as well as emerging methods for improving three-dimensional optical sectioning microscopy

Reviews the basics of FRET (fluorescence resonance energy transfer) and considers its new use for investigating protein complexes.

The text also introduces those emerging nonfluorescence microscopy methods that can actually exert mechanical forces to trap and move a variety of objects ranging from beads to living cells and cellular organelles. Combining this technique with fluorescence microscopy provides an unparalleled ability to manipulate and visualize biological samples.